πŸ”¬ Mutagenesis Primer Design

Site-directed Mutagenesis Primer Design

Automatically design primers for site-directed mutagenesis by PCR. The tool calculates Tm, optimizes primer length, and performs quality checks. Four modes are supported: point mutation, amino-acid substitution, deletion, and insertion.

β‘  Enter Template Sequence
Template DNA sequence (5'β†’3' sense strand)
Length: 0 bp   GC%: β€”
β‘‘ Select Display Mode and Upload
β‘£ Mutation Type and Settings
Mutation Type
Nucleotide substitution/deletion parameters (up to 7 positions can be specified at once)

Click a base in the sequence viewer to add it automatically. Select β€œ-” as the mutant base to delete that nucleotide.

πŸ“Š Design Summary
🧬 Designed Primers
Position of primers on the double-stranded template
πŸ–₯ GeneQuick is an intuitive plasmid DNA editing app.

Use the GeneQuick app to check mutations, run automatic sequence analysis, and manage primer lists.

Free Download β†’