DNA and protein analysis
made easier in your browser.

Remove spaces, line breaks, numbers, FASTA headers, and other unwanted characters from copied sequences to prepare them for analysis.

Convert T to U or U to T. Uppercase/lowercase letters and separators are handled automatically.

Generate the reverse complement of a DNA sequence. Useful for primer design, cloning, and checking antisense strands.

Translate DNA/RNA sequences based on the codon table. Supports reading frames, automatic ORF detection, and one-letter/three-letter amino acid notation.

Back-translate amino acid sequences into DNA sequences using species-specific codon usage tables or manual codon selection.

Search by gene name, symbol, or accession number to retrieve and copy mRNA and protein sequences from NCBI in real time.

Design PCR primer candidates with real-time Tm, GC%, and quality checks using the nearest-neighbor method.

Design mutagenesis primers for introducing point mutations, amino acid substitutions, insertions, deletions, and more.

Fetch ORF and RefSeq mRNA from gene name and organism, then launch Primer-BLAST with all parameters pre-filled in one click.

Compare and access CRISPick and CRISPOR — the two leading gRNA design tools.

Optimize a DNA sequence based on the codon usage of a selected organism and show changed positions and GC% changes.

Select from blastn, blastp, blastx, tblastn, and tblastx to launch NCBI BLAST directly with the right program for your query.

Search for major restriction enzyme sites in a DNA sequence and list cutting patterns, overhangs, and fragment sizes.

Calculate G+C content and display the GC profile, base composition, and high-/low-GC regions of a DNA sequence.

Run Clustal Omega multiple sequence alignment in the browser. Supports iterative refinement and phylogenetic tree visualization. All data stays local.

Run multiple sequence alignment using ClustalW-style parameters, with support for matrix selection and gap adjustment.

Apply shading to identical and similar residues in multiple sequence alignments, with adjustable display width and color schemes.

A simple browser-based MAFFT tool supporting Fast progressive and Accurate progressive modes.

Calculate the number and percentage of each amino acid in a protein sequence and display the results as graphs and tables.

Calculate protein molecular weight, isoelectric point, and amino acid composition from an amino acid sequence.

Calculate hydrophobicity and display candidate transmembrane regions as Primary or Secondary candidates.

Detect N-X-S/T motifs (X ≠ P) and check atypical motifs or lower-efficiency candidates affected by proline.