DNA and protein analysis
made easier in your browser.

Remove spaces, line breaks, numbers, FASTA headers, and other unwanted characters from copied sequences to prepare them for analysis.

Convert T to U or U to T. Uppercase/lowercase letters and separators are handled automatically.

Generate the reverse complement of a DNA sequence. Useful for primer design, cloning, and checking antisense strands.

Translate DNA/RNA sequences based on the codon table. Supports reading frames, automatic ORF detection, and one-letter/three-letter amino acid notation.

Back-translate amino acid sequences into DNA sequences using species-specific codon usage tables or manual codon selection.

Design PCR primer candidates with real-time Tm, GC%, and quality checks using the nearest-neighbor method.

Design mutagenesis primers for introducing point mutations, amino acid substitutions, insertions, deletions, and more.

Calculate G+C content and display the GC profile, base composition, and high-/low-GC regions of a DNA sequence.

Optimize a DNA sequence based on the codon usage of a selected organism and show changed positions and GC% changes.

Search for major restriction enzyme sites in a DNA sequence and list cutting patterns, overhangs, and fragment sizes.

Run multiple sequence alignment using ClustalW-style parameters, with support for matrix selection and gap adjustment.

Apply shading to identical and similar residues in multiple sequence alignments, with adjustable display width and color schemes.

A simple browser-based MAFFT tool supporting Fast progressive and Accurate progressive modes.

Calculate the number and percentage of each amino acid in a protein sequence and display the results as graphs and tables.

Calculate protein molecular weight, isoelectric point, and amino acid composition from an amino acid sequence.

Calculate hydrophobicity and display candidate transmembrane regions as Primary or Secondary candidates.

Detect N-X-S/T motifs (X ≠ P) and check atypical motifs or lower-efficiency candidates affected by proline.